Our Enzymatic Injection Molding Technology
Revolutionizing DNA Synthesis
At Moligo we have developed a proprietary and patented Enzymatic Injection Molding Technology (EIMT) for large-scale production of long ultrapure single-stranded DNA (ssDNA) molecules.
Instead of “printing” DNA molecules as chemical approaches or other enzymatic DNA synthesis methods do, we mass produce DNA molecules from sequence-verified “production templates” (our molds), using a PCR free in-vitro enzymatic technology.
The most common DNA synthesis methods are based on chemical approaches, which produce DNA with significant limitations in length, purity, scalability, and complexity. Fermentation-based ssDNA production, another common method, also has disadvantages because it produces DNA with extra viral and bacterial sequences as scar sequences of the sequence of interest.
Our EIMT technology is based on scalable isothermal in vitro enzymatic reactions, which guarantee the industrial-scale synthesis of ultrapure, 10 Kb long ssDNA molecules, with any complexity and, when required, internal chemical functionalities.
How Enzymatic Injection Molding Technology Works
Superior ssDNA for your technologies and therapies
-
![]()
Purity
Highly pure ssDNA is guaranteed by the fidelity of our polymerases, which have an error rate of 1 in 10 million bases. Additionally, we reduce the risk of contamination from endotoxins because our in vitro method does not use bacteria or viruses to produce DNA. Purity is key for many DNA- based therapeutics and diagnostics.
-
![]()
Length
We can synthesize ssDNA molecules over 10 Kb. DNA length matters for the development of gene therapies that require long therapeutic genes, such as Cystic Fibrosis and many muscular dystrophies, and to overcome current size limitations of AAV-based therapies.
-
![]()
Complexity
DNA sequences with any complexity including high CG content regions, inverted repeats and strong secondary structures, which are very common in human and microorganism genomes. High complexity is currently limiting the development of new gene-based therapies and technologies.
-
![]()
Scalability
The efficiency of our enzymes combined with our technogy’s use of isothermal reactions enable industrial scale production of DNA. Today we are producing grams of ssDNA for biotech and pharma companies.
-
![]()
Multifunctionality
At Moligo we can synthesize ssDNA with internal chemical functionalities that can help tune the properties of these molecules, making them more customized to the specific downstream applications.
Moligo is supported by
